Control of Metabolic Homeostasis by Stress Signaling Is Mediated by the Lipocalin NLaz (original) (raw)
Figure 1
JNK is required for maintenance of metabolic homeostasis.
(A–E) Comparison of hemizygous hep1 males (hep1/y) to wild type OreR males. (A–C) Carbohydrate and lipid content in homogenates prepared from populations of 10 flies prior to and after 6, 24, and 30 hours of wet starvation. All measurements were normalized to the average weight of a single fly in its population. (A) Glucose. (B) Glycogen. (C) Triglycerides. (D) Real time PCR results from cDNA prepared from starved adult males collected 6, 24, and 30 hours after wet starvation. Levels of PEPCK are compared in starved conditions to fed controls (0 hours). All transcripts are normalized to actin5C. (E,F) Percent of male flies surviving after prolonged dry starvation. Population sizes were (E) OreR: n = 151; hep1: n = 103. Lifespan differences are statistically significant (p<0.001, log rank test). (F) DaG4/+: n = 179; BskRNAi/+;DaG4/+: n = 138. p<0.001, log rank test. (F, inset) Real time PCR on cDNA prepared from BskRNAi/+;DaG4/+ larvae. Levels of Bsk are compared to DAG4/+ controls. All transcripts are normalized to rp49.