Tor1/Sch9-Regulated Carbon Source Substitution Is as Effective as Calorie Restriction in Life Span Extension (original) (raw)
Figure 3
Expression of the glycerol biosynthesis genes.
(A) Schematic representation of glycerol metabolism. For illustration purpose, genes up- or down-regulated more than 20%, compared to wild type (DBY746) in all three long-lived mutants, are labeled in red or green, respectively. (B) Fold change in expression levels of glycerol biosynthetic genes in _sch9_Δ, _tor1_Δ, and _ras2_Δ mutants compared to wild type at day 2.5 (for complete microarray data, see Table S2 and Table S6). Data were expressed in fold change, WT = 1. Gene expression levels that are 20% higher or lower than wild type cells are marked in red or green. 1 Predicted PDS motif: AWAGGGAT; 2 predicted STRE motif: ARGGGG; 3 predicted STRE motif: AGGGG. (C) Real time quantitative PCR analysis of GPD1 mRNA level _sch9_Δ and _sch9_Δ _gis1_Δ cells at day 2.5. Data represent mean and SEM, n = 4. * p<0.05, _t-_test, two-tailed, _sch9_Δ vs. WT.