Tor1/Sch9-Regulated Carbon Source Substitution Is as Effective as Calorie Restriction in Life Span Extension (original) (raw)
Figure 5
Deletion of glycerol biosynthesis genes reverses life span extension and stress resistance associated with Sch9 deficiency.
(A) Glycerol concentration in the medium. Data represent mean and SEM of 4 cultures analyzed. * p<0.05, ** p<0.01, unpaired _t_-test, two tailed, _sch9_Δ _vs. rhr2_Δ _sch9_Δ. (B) Life span of wild type (DBY746), _sch9_Δ, _rhr2_Δ, and Sch9-deficient mutants lacking Rhr2. Glycerol (1%, final concentration) was added to the one day-old _rhr2_Δ _sch9_Δ culture. Data represent mean and SEM of 4–5 cultures analyzed. (C) Day3 cells were exposed to heat shock (55°C for 105 min) or H2O2 (150 mM for 60 min). Strains shown are wild type (DBY746), _rhr2_Δ, _sch9_Δ, and _rhr2_Δ _sch9_Δ. (D) Ethanol concentration in the medium. Data represent mean and SEM, n = 3. Ethanol in _sch9_Δ culture at day 5 was at the lower detection limit of the assay. (E) Life span of wild type (BY4741), _sch9_Δ, and Sch9-deficient mutants lacking Gpd1, Gpd2, or Rhr2. Data represent mean and SEM of 3 experiments. (F) Heat shock (55°C) and oxidative stress (H2O2, 500 mM, 60 min) resistance of day 3 mutants lacking glycerol biosynthesis genes (in the BY4741 genetic background).