synaptojanin1 Is Required for Temporal Fidelity of Synaptic Transmission in Hair Cells (original) (raw)
Figure 4
High frequency stimulation resulted in time-dependent reduction of the number of spikes in synj1Q296X afferent neurons.
A) Spikes recorded from a wild-type (black traces) and synj1Q296X (grey traces) larva in response to 20 Hz stimulation. Shown is an overlay of 60 consecutive sweeps from an individual larva. B\) Box plot summarizing the spike rate (spikes per second) for 60 seconds of 20 Hz stimulation (black bars, wild-type; grey bars, synj1Q296X; includes larvae in A. C) Spikes recorded from a wild-type (black traces) and synj1Q296X (grey traces) larva in response to 60 Hz hair-cell stimulation. Shown are 60 consecutive sweeps overlaid from a single larva. D) Box plot summarizing the reduction in spike rate of synj1Q296X (grey bars) during 60 seconds of 60 Hz stimulation compared to wild-type neurons (black bars). Following 15 minutes of sustained stimulation the synj1Q296X mutant spike rate was reduced compared to both the initial rate and the wild-type rate.