Evolutionary Breakpoints in the Gibbon Suggest Association between Cytosine Methylation and Karyotype Evolution (original) (raw)
Figure 1
Analysis of gibbon specific segmental duplications.
(A) Fluorescence in situ hybridization (FISH) experiments on NLE nuclei and metaphases using gibbon BACs spanning breakpoints which overlap with gSD. The fluorescent signals show a pattern typical of repeated sequences; (B) Images from the Array-CGH experiment using gibbon (test) versus human (reference) genomic DNA. Human chromosomes 2 and 3 are shown; duplications are represented in green and deletions in red. The duplications were validated by FISH on metaphases and nuclei of both human and gibbon using as probes the human BACs from the 32Kset. Duplicated regions present a higher depth of coverage of Trace Archives reads on the human genome as illustrated in the lateral panels.