Methylated H3K4, a Transcription-Associated Histone Modification, Is Involved in the DNA Damage Response Pathway (original) (raw)
Figure 5
Replication defects in _set1_Δ cells.
(A) Serial ten-fold dilution growth test of strains with the relevant genetic setup on plates with 50mM HU. Strains used were YZS267, DFY017, DFY038, DFY039, DFY024, DFY026 and MT0-73. (B) Viability curves of the indicated strains exposed to HU for the indicated time. Strains used were wt (DFY024), _set1_Δ (DFY026), _arp8_Δ (DFY030), _mec1_Δ (DFY044). (C) wt (DFY024), _set1_Δ (DFY026)or _rsc30_Δ (DFY029) cells were arrested in G1 phase of the cell cycle and released into a synchronous S-phase in the presence of 200 mM HU for 60 min. The HU block was removed by washout and cell cycle progression was analyzed by FACS. Time 0 min represents the release of cells into rich medium plus Nocodazole following the HU treatment. (D) Viability of strains with indicated genotype and derived from strain DFY032 were grown in liquid media and ten-fold serial dilutions were plated onto YEPD control plates or onto a plate containing bleomycin, as indicated.