DNMT3L Modulates Significant and Distinct Flanking Sequence Preference for DNA Methylation by DNMT3A and DNMT3B In Vivo (original) (raw)
Figure 1
DNMT3A methylation patterns are characterized by reproducible and symmetrical high and low efficiency methylation sites.
A. This panel represents the DNA methylation patterns deposited by DNMT3A at the pBR top strand region as determined by bisulfite methylation sequencing. Each horizontal line corresponds to an independent DNA molecule while each vertical line corresponds to one of the 48 CpG sites in this region. Black squares indicate CpG methylation while white squares represent absence of methylation. The arrows indicate the two sites with the greatest and least methylation frequencies, respectively, along with their corresponding methylation frequencies. B. Two independent in vivo samples were analyzed for their DNA methylation patterns and the CpG sites were ranked according to their methylation efficiency. The ranks observed at each of the 48 CpG sites were then plotted against each other in one sample versus another. Overall, a good correlation exists between methylation ranks, indicating that the methylation patterns for DNMT3A are reproducible. C. The individual methylation efficiency at each CpG site along the pBR322 region are plotted for both top and bottom DNA strands. The patterns observed are clearly symmetrical across DNA strands.