DNMT3L Modulates Significant and Distinct Flanking Sequence Preference for DNA Methylation by DNMT3A and DNMT3B In Vivo (original) (raw)
Figure 7
Stimulation by DNMT3L is observed primarily on newly replicated DNA strands.
The overall methylation efficiencies (in percentages) observed on both strands at the pBR322 and Hygro regions are indicated in black bars for DNMT3A (panel A) and DNMT3B (panel B). The number of independent DNA molecules taken into account to calculate the average methylation efficiencies varied from 36 molecules to 120 per sample. In the presence of DNMT3L, the data were broken down between dcm+ (grey bars) and dcm− (checkered grey bars) DNA strands, as indicated. Whether the stimulation afforded by DNMT3L was significant or not was determined by a one tailed Student t-test and is indicated graphically (‘no’, not significant; ‘***’ P-value<0.001; ‘**’ P-value between 0.001 and 0.01; ‘*’ P-value between 0.01 and 0.05). While DNMT3L-mediated stimulation of DNA methylation is readily observed at all tested regions on newly synthesized DNA molecules (dcm−), it is not observed in the majority of cases for dcm+ molecules.