Actin Depolymerizing Factors Cofilin1 and Destrin Are Required for Ureteric Bud Branching Morphogenesis (original) (raw)
Figure 3
Efficiency of _Hoxb7/CreGFP_-mediated deletion of Cfl1 as evaluated by Cofilin1 antibody staining.
Calbindin (green) was used to visualize Wolffian duct epithelium in (A–D). (A–B'), cofilin1 (red) and calbindin (green) in E10.5 Wolffian duct (sections through the expanded region that will give rise to the UB). Cofilin1 is expressed in every cell in both ureteric bud and metanephric mesenchyme, and seems slightly enriched in UB epithelial cells. At E10.5, there is no detectable difference in cofilin1 protein amount or localization between control (Dstn+/−) and Hoxb7/CreGFP; Cfl1F/F; Dstn−/− kidneys. (C–D'), At E12.5, cofilin1 localization in the Dstn+/− control is similar to that at the earlier stages. However, Hoxb7/CreGFP has efficiently deleted the gene in the UB, as the protein is absent in ureteric epithelium of Cfl1F/F kidneys. Arrows point to ureteric buds, scale bar 50 µm.