Actin Depolymerizing Factors Cofilin1 and Destrin Are Required for Ureteric Bud Branching Morphogenesis (original) (raw)
Figure 6
Migration assay for primary ureteric bud cells reveals defects in epithelial cell movement in Cfl1;Dstn double mutant.
A scratch was introduced 2 days after plating the isolated ureteric buds (0 h, A and C) and followed for 24 h (B and D). Control _Dstn−/−_cells (A–B) had completely filled the gap produced by the scratch (N = 12 cultures), while double mutant cells (C–D) were impaired in their movement (N = 5 cultures). Scale bars 200 µm.