Actin Depolymerizing Factors Cofilin1 and Destrin Are Required for Ureteric Bud Branching Morphogenesis (original) (raw)
Figure 8
Cfl1 gene expression is not regulated by GDNF/Ret signaling in developing kidney.
(A–A'), Cfl1 in situ hybridization in E11.5 wild-type kidney cultured with control, BSA-soaked bead. A' shows a higher magnification of the boxed area in A. mRNA is detected both in metanephric mesenchyme (m) and ureteric epithelium (u). (B–B'), Similar expression levels of Cfl1 in kidneys cultured with GDNF-soaked beads, which induce enlargement of ureteric tips and extra budding from Wolffian duct (arrows). B' shows a higher magnification of the boxed area in B. (C), Cofilin1 antibody staining of Ret+/− (control) E10.5 embryo, where Wolffian duct shows epithelial thickening as a hallmark of early ureteric bud outgrowth. (D), Normal expression of Cofilin1 protein (red) in _Ret_−/− Wolffian duct epithelium at E10.5. (E,F) F-actin localization in control and _Ret_−/− kidneys at E10.5. Scale bars 100 µm for A–B, 50 µm for C–F.