Altered DNA Methylation in Leukocytes with Trisomy 21 (original) (raw)
Figure 3
Quantitative MS-Pyroseq showing recurrent hypomethylation in the TMEM131 internal promoter region, TCF7 upstream region, and CD3Z promoter region, and hypermethylation in the EIF4E, SH3BP2, SUMO3, and PLD6 promoter regions, in DS compared to normal PBL.
Genomic DNAs were bisulfite converted and subjected to PCR followed by MS-Pyroseq. The values for percent methylation are averaged over multiple CpG dinucleotides in each region, as listed in Table S7. Mean values for methylation and the p-values from logistic regression adjusting for age are in Table 1. DS, total PBL from adults with DS; Control, total PBL from normal adults; CB, total leukocytes from normal umbilical cord blood; PBMC, normal adult peripheral blood mononuclear cells; PMN, normal adult peripheral blood polymorphonuclear leukocytes.