Altered DNA Methylation in Leukocytes with Trisomy 21 (original) (raw)
Figure 4
Altered mRNA expression of TMEM131, TCF7, and NPDC1 in DS versus normal PBL.
A, Expression of TMEM131 short isoform mRNA measured by Q-PCR. The difference between DS and normal controls is highly significant (T-test, p<0.0001). B, Expression of TCF7 mRNA measured by Q-PCR. The difference between DS and normal controls is highly significant (T-test, p = .00012). C, Expression of NPDC1 mRNA measured by Q-PCR. The difference between DS and normal controls is highly significant (T-test, p<0.0001). The Q-PCR primers are listed in Table S7. The TMEM131 Q-PCR primers are specific for the short mRNA isoform; the TCF7 Q-PCR primers amplify both major isoforms of TCF7 mRNA. The NPDC1 Q-PCR primers amplify all major isoforms.