Altered DNA Methylation in Leukocytes with Trisomy 21 (original) (raw)
Figure 6
Differential DNA methylation in the CARD15/NOD2 gene in DS versus normal T-cells and induction of NOD2 mRNA by 5aza-dC.
A, Fractional methylation values from the Infinium assays showing differential methylation at cg26954174 in the NOD2 promoter region in DS versus control T-cells. B, Bisulfite sequencing showing relative hypomethylation in the (non-CGI) promoter region of NOD2 in DS T-cells. The map of the NOD2 promoter region shows the location of the Infinium probe (grey bar above the line, cg26954174); the region subjected to bisulfite sequencing is shown by the grey bar below the line. C, Increased expression of NOD2 mRNA in PBMCs exposed to 5aza-dC at the indicated concentrations for 3 days. The short term cultures of PBMCs were grown in the presence of PHA to induce a polyclonal proliferation of T-cells.