Season of Conception in Rural Gambia Affects DNA Methylation at Putative Human Metastable Epialleles (original) (raw)
Figure 1
Two-tissue MSAM screen.
(A) Agarose gel images showing MSA product from PBL and HF genomic DNA of the 8 individuals included in the screen. MSA amplifies methylated SmaI/XmaI intervals ranging from <100 bp to 2 kb. (B) Results of the MSAM screen at chromosome 2 for one pair of PBL and HF interindividual cohybridizations. Each bar represents the average of all probes within a single SmaI/XmaI interval on chromosome 2. The y axis is a log10 transformation of the P value of the interindividual signal ratio. Red and blue bars represent positive and negative interindividual differences, respectively, with P<10−4. SmaI/XmaI intervals showing concordant interindividual differences in PBL and HF are ME candidates; the location of the hit at PAX8 is indicated.