Global Analysis of the Impact of Environmental Perturbation on cis-Regulation of Gene Expression (original) (raw)
Figure 2
Global response on gene expression upon environmental perturbation.
The significance of the treatment-induced effects at two time points, 2h (left panel)and 24h (right panel), on global gene expression assessed by AffymetrixU133+2 arrays (A) and IlluminaRef8 beadarrays (B) in two individuals, respectively, was determined by the Cyber-t test using three biological replicates in BMP-2 (top), DEX (middle) and PGE2 (bottom) treated versus control samples, respectively. The results from the statistical tests are combined with the magnitude of the change in gene expression following perturbation and visualized in Volcano plots with significance (−log10 P value) versus fold change (log2) on the y – and x-axes, respectively. Lines indicate genes whose expression levels were significantly (FDR adjusted P<0.05) changed more than 2-fold.