Global Analysis of the Impact of Environmental Perturbation on cis-Regulation of Gene Expression (original) (raw)
Figure 5
Global effect of environmental perturbation on allelic _cis_-regulation.
(A) Treatment specific changes in AE within an individual (left panel). A total of 224,944 expressed three SNP windows measured in both DEX samples and in three other conditions were available across four individuals. The x-axis shows magnitude of AE (|Δhet ratio|) in reference DEX window correlated against magnitude of AE observed in independent DEX measurement on y-axis, against AE observed in BMP-2 treatment, against PBS or PGE2. The Pearson correlation (R) for each pair-wise comparison is shown and demonstrates that treatment contributes slightly to differential _cis-_regulation observed by the genome-wide AE test. (B) Interindividual, treatment independent variation in allelic expression (upper right panel). A total of 27,836 identical informative three SNP windows were measured between individuals upon DEX treatment. The Pearson R is considerably lower than for within sample comparisons indicating that most of the AE variation between individuals is driven by determinants shared between these cells and independent of treatment. (C) Interindividual, treatment dependent variation in AE (right panel). A total of 159,926 identical informative three SNP windows were available for correlating DEX treatment to any of the three other treatments between individuals. Correlations between individuals across treatments are not significantly different from within treatment correlation between individuals shown in (B), indicating that the treatment specific effects observed within individual (A) are sufficiently weak to be globally unnoticeable due to much stronger correlation of AE to interindividual differences.