Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium (original) (raw)
Figure 3
Associations between chromatin and cell type–specific expression patterns.
(A) Overall correlation between histone methylation (K4, K27) and gene expression in CD44+ progenitors from sample 1. Box plot shows distribution of gene expression of corresponding genes in each category. Red bar: median, box: interquartile ranges and whisker; most extreme value within 1.5 times of box length. (B) Bar chart shows the correlation between cell type–specific K27 enrichment and gene expression patterns. Blue and red: K27-enriched only in CD24+ and CD44+ cells, respectively, yellow and gray: K27 enriched in both and neither cell type. (C) Differences in K27 patterns between CD24+ and CD44+ cells are consistent in three individual samples and distinct from that of K4 profiles. Heatmap depicting unsupervised clustering of histone modification patterns of genes highly expressed in CD44+ (656 genes) and CD24+ (435 genes) cells. Blue color indicates the level of enrichment for the indicated histone modification based on the ranking of ChIP-Seq read counts for each gene in each sample. (D) Differentially expressed genes that are enriched (K27+) or not enriched (K27-) for K27 were analyzed for relative enrichment with the indicated protein classes (left panel) and for relative connectivity (right panel). X-axes indicate –log10 p-values for enrichment with the listed protein classes (left panel) and the number of overconnected objects, defined as proteins with higher than expected number of interactions, in each functional category within each group (right panel), respectively.