Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium (original) (raw)
Figure 5
Changes in chromatin state and cell type–specific gene expression patterns.
(A) Number of genes for each of the four possible chromatin states (i.e., bivalent – purple, K4 only – orange, K27 only – green, and neither – gray) in the indicated three cell types. (B) Potentially interesting differences in chromatin patterns. Bar chart shows associations between changes in chromatin-state and gene expression patterns. Each row indicates the type of chromatin-state change and the number of genes in each category. Blue and red: genes highly expressed in CD24+ and CD44+ cells, respectively (≥2-fold change). Yellow and gray: genes with ≤2-fold difference between CD24+ and CD44+ cells and with low/no detectable expression in either cell type, respectively. (C) Representative examples of genes in each category. ChIP-Seq tag counts for K4 and K27 modification in hES, CD44+, and CD24+ cells are shown. Total aligned tag count was scaled to 10 million and tag counts were averaged over a 10 bp window. (D) Functional enrichment analysis of genes within each chromatin pattern category (left panel) and the number of overconnected objects in each functional category within each group (right panel). X-axis indicates –log10 p-values for enrichment with the indicated protein class (left panel) and the number of overconnected objects (right panel), respectively. Definitions are the same as described in Figure 3D.