Hunger Artists: Yeast Adapted to Carbon Limitation Show Trade-Offs under Carbon Sufficiency (original) (raw)
Figure 4
Expression Changes Common to Evolved Clones.
Significance analysis was performed as a 2-class SAM between all evolved clone data and all ancestral data for the three conditions. Prior to clustering the data were normalized to the ancestor as for Figure 3. Data from [51] represents a time course of a constitutive Ras2G19V allele induced by the GAL10 promoter. mRNA abundances at 0, 20, 40, and 60 min were measured on an Affymetrix platform and normalized for our purposes to 0 min (log2(20 min/0 min), etc.). Data from [52] are relative mRNA abundances over a time course (0, 15, 30, 60 min) of rapamycin (rap) treatment normalized to 0 min (log2(15 min/0 min), etc.). Data from [53] are relative mRNA abundance of rapamycin treated wild type cells versus wild type (log2(rapamycin/no treatment)). Each row represents a gene, and grey indicates missing data. Clustered raw data are available in Dataset S2.