FUS and TARDBP but Not SOD1 Interact in Genetic Models of Amyotrophic Lateral Sclerosis (original) (raw)
Figure 4
FUS rescues the motor phenotype induced by Tardbp KD, but not vice-versa.
A) Motor phenotype was assessed both by time frames obtained from video recordings of the TEER as well as B) immunohistochemical labeling of the axonal projections to determine UAL in motor neurons. The analysis shows that both expression of WT TARDBP and WT FUS can rescue the motor phenotype caused by KD of Tardbp (i) and Fus (ii) respectively. Furthermore, WT FUS is able to rescue the motor phenotype induced by KD of Tardbp (iii), but WT TARDBP does not rescue the motor phenotype caused by KD of Fus (iv). Similarly, WT SOD1 is unable to rescue Fus (v) and Tardbp (vi) KD phenotypes. Arrowheads represent length to the first axonal branching (UAL). Scale bar: 40 µm.