MicroRNA Expression and Regulation in Human, Chimpanzee, and Macaque Brains (original) (raw)
Figure 5
miRNA transfection effects in two cell lines.
The dark grey bars depict the inhibition ratio of conserved miRNA targets, predicted using the TargetScan algorithm. The light grey bars depict the inhibition ratio of non-target genes. Inhibition ratio was calculated as the number of genes down-regulated 24 hours after miRNA transfection, divided by the number of not-down-regulated genes. For each gene, the miRNA transfection effect was calculated as a ratio of mRNA expression level 24 hours after miRNA transfection, divided by mRNA expression level 24 hours after transfection with negative controls (Materials and Methods). The significance of difference between target and non-target inhibition ratios estimated using Fisher's exact test is shown above the bars: *** - p<0.001; ** - p<0.01; * - p<0.05.