Foxn1 Regulates Lineage Progression in Cortical and Medullary Thymic Epithelial Cells But Is Dispensable for Medullary Sublineage Divergence (original) (raw)
Figure 2
Functional deficit in Foxn1R/R TEC.
Morphological and phenotypic analysis of 6 week old Foxn1R/R thymi. (A) H&E staining showing frequent large medullary cysts in Foxn1R/R thymi. (B) Immunostaining with anti-cytokeratin 5 (K5) and anti-cytokeratin 8 (K8) showing overtly normal cortical and medullary compartments in Foxn1R/R thymi; note the normal morphology of cortical and medullary TEC. (C-E) Flow cytometric analysis of 6 week old wild-type or Foxn1R/R thymocytes with (C) anti-CD4-PE, anti-CD8-FITC, (D) anti-CD44-APC and anti-CD25-PE after gating on Lin- cells (Lin = anti-CD3, anti-CD4, anti-CD8, anti-CD11b, anti-CD11c, Ter119, NK1.1, Gr-1), or (E) B220 after gating on DN1 cells in (D). (E) red lines show Foxn1R/R; blue lines, WT. (F) Flow cytometric analysis of E13.5 or E15.5 fetal thymic primordia with anti-CD45-APC Cy7; anti-CD25-PE, and anti-CD44-APC. PN, postnatal; WT, wild type. (E) shows pooled data from three 6 week old mice, n = 1. All other data are representative of at least three separate analyses.