Population Genomic Scan for Candidate Signatures of Balancing Selection to Guide Antigen Characterization in Malaria Parasites (original) (raw)
Figure 7
Transcript profiles of the six _msp3_-like genes in P. falciparum clinical and laboratory isolates grown to schizont stages.
(A) Genomic loci of the six _msp3_-like genes on parasite chromosome 10 (nomenclature and map is based on 3D7 genome sequence version 2.1). Quantitative RT-PCR was based on non-polymorphic sequences (oligonucleotide primers and probes are given in Table S4). (B) Variation in relative transcript levels for the six _msp3_-like genes among 45 Gambian clinical isolates. Relative transcript levels for each gene in each isolate are normalized as a proportion of the sum for all six genes within the isolate. (C) Variation in transcript levels of the genes among 11 diverse laboratory-adapted cultured isolates. (D) Cluster analysis of expression profiles in the 45 clinical isolates and 11 laboratory-adapted isolates. Laboratory isolates are interspersed with the clinical isolates throughout, except for a divergent cluster of only clinical isolates on the right of the figure expressing little or no transcript of the msp6 gene PF10_0346 (including one isolate that abundantly expressed the h103/msp11 gene PF10_0352).