Histone Methyltransferase DOT1L Drives Recovery of Gene Expression after a Genotoxic Attack (original) (raw)
Figure 7
Pre-initiation complex assembly and chromatin modification after UV irradiation.
(A) Determination of the relative mRNA expression level of DHFR gene in MEFWT and MEFDOT1L after UV irradiation (10 J/m2) performed after reverse transcription with random hexameres. The values are the means of three independent experiments (± SD). AUC was determined and p-value was extrapolated using t-test. (B–F) Time-dependent occupancy of RNA Pol II (B), TFIIB (C), H4Ac (D), H3K9me2 (E) and H3K79me2 (F) at the promoter of the DHFR gene following UV irradiation (10 J/m2). Soluble chromatin was prepared from MEFWT and MEFDOT1L cells at indicated time points after UV-C treatment and subjected to ChIP assay using the indicated antibodies. Real-time PCR using specific primers was performed to test the relative enrichment at the proximal promoter of the DHFR gene. The results are expressed as folds of enrichment relative to the untreated cells. The values are the means of a triplicate experiment (± SD). P-value was extrapolated using t-test (**<0.05, ***<0.005).