Limiting of the Innate Immune Response by SF3A-Dependent Control of MyD88 Alternative mRNA Splicing (original) (raw)
Figure 2
Inhibition of SF3A1 or SF3B1 diminishes PAM3CSK4-induced inflammatory cytokine production.
Cells were transfected with the indicated siRNAs (either Sf3a1, Sf3b1, or either of two different control non-targetting siRNAs) and treated with PAM3CSK4 where indicated (B) or in all cases (A,C). Subsequently IL-6 protein (A), IL-6 mRNA (B), or TNFα protein (C) levels were monitored. In panel B, RNA levels of 1 are defined as IL-6 mRNA levels in the presence of control siRNA treatment in the absence of PAM3CSK4.