Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila (original) (raw)
Fig 8
Jigr1 upregulation is responsible for premature neuroblast differentiation in miR-92 –/– flies.
(A) Neuroblast number in third instar larval brains of the indicated genotypes at 120 hr ALH. w 1118 (n = 17); miR-92 4/4 (n = 15); miR-92 1/4 (n = 20); Def(3R)BSC321/miR-92 4 (n = 17); Insc-GAL4/UAS-Jigr1 RNAi ; miR-92 1/4 (n = 14). (B) The size of Adult brains of indicated genotypes. w 1118 (n = 10); miR-92 4/4 (n = 10); miR-92 1/4 (n = 8); Def(3R)BSC321/miR-92 4 (n = 12); Insc-GAL4/UAS-Jigr1 RNAi ; miR-92 1/4 (n = 8). (C) Immunostaining of third instar larval brains of wild type (left panel), Insc-GAL4/UAS-jigr1 (middle panel) and Insc-GAL4/UAS-jigr1-RNAi (right panel) flies. Green: Jigr1. Red: Miranda. Scale Bar: 20 m. (D) Percentage of BrdU_+_ neuroblasts in wild type control (n = 12), Insc-GAL4/UAS-jigr1 (n = 15) and Insc-GAL4/UAS-jigr1-RNAi (n = 9) larval brains. (E) Immunostaining of _Insc-GAL4/UAS-jigr1_brains with Prospero (green) and Dpn (red) at 120 hr ALH. (F) Adult brain volume of wild type control (n = 10) and Insc-GAL4/UAS-jigr1 (n = 9) flies. (G) Working model summarizing the expression and the role of miR-92 in larval neuroblasts. Statistical significance was determined by One-Way ANOVA. Values are mean ± s.e.m. in all graphs. *: p < 0.05, **: p < 0.005, ***: p < 0.001, ****: p < 0.0001.