Allelic Variation of Cytochrome P450s Drives Resistance to Bednet Insecticides in a Major Malaria Vector (original) (raw)
Fig 6
Substrate depletion and kinetic profiles of mutant CYP6P9b recombinant proteins.
(A and B) are percentages of depletion of 20μM deltamethrin and permethrin by CYP6P9b mutants, respectively. Results are average of three replicates (n = 3) compared with negative control (-NADPH). (C) Michaelis-Menten plots of mutant CYP6P9b proteins metabolism of permethrin; (D) 4D plot of the kinetic constants and catalytic efficiencies of mutant CYP6P9b proteins for metabolism of permethrin.