Adaptation to High Ethanol Reveals Complex Evolutionary Pathways (original) (raw)
Fig 2
Evolved populations are more ethanol tolerant.
Evolved populations from different reactors show increased fitness in EtOH. Fitness was determined for population samples of each reactor after 40 generations (blue) and 200 generations (red). Fitness is expressed relative to the ancestral strain of each reactor (haploid for reactor 1–2; diploid for reactor 3–4 and tetraploid for reactor 5–6). Data represent the average of three independent measurements, error bars represent standard deviation. Populations with mutator phenotypes showed higher standard deviations. This might be due to the large supply of mutations generated by these mutators, which could affect mutation-selection balance.