Whole-Organism Developmental Expression Profiling Identifies RAB-28 as a Novel Ciliary GTPase Associated with the BBSome and Intraflagellar Transport (original) (raw)
Fig 4
BBSome-dependent recruitment of activated RAB-28 to the periciliary membrane.
(A, B) Representative images of amphid and phasmid cilia from N2 wild type (A) and bbs-8(nx77) mutant (B) worms expressing GFP-tagged RAB-28(WT), RAB-28(GDP) and RAB-28(GTP) reporters. All reporters are driven by the endogenous rab-28 gene promoter. Kymographs and kymograph schematics derived from time-lapse imaging of GFP signals in phasmid cilia. Phenotypes summarised in cartoons. Large phasmid images are placed on black backgrounds. m; middle segment, d; distal segment. pcmc; periciliary membrane compartment (also denoted by arrow). Scale bars; 2 μm and 5 μm (low magnification phasmid images).