rahu is a mutant allele of Dnmt3c, encoding a DNA methyltransferase homolog required for meiosis and transposon repression in the mouse male germline (original) (raw)
Fig 4
DNMT3C is a putative DNA methyltransferase with similarity to DNMT3B.
(A) Schematics of DNMT3C and DNMT3B showing the location of conserved domains and the rahu mutation (asterisk). (B) Cladogram of Clustal Omega aligned human and mouse DNMT3 family sequences rooted with HhaI. (C) Motif IX in Clustal Omega aligned sequences showing the location of the rahu mutation (asterisk). DNMT3L proteins do not contain Motif IX. Amino acids identical to those in DNMT3C are shaded gray. Amino acid positions refer to DNMT3C. (D) Homology-based model of DNMT3C carboxy-terminal domain (cytosine methyltransferase domain and the preceding four amino acid residues) with E692 depicted in red. (E) Crystal structure of the DNMT3A carboxy-terminal domain dimer (PDB ID:2QRV), with monomers depicted in two shades of blue. The DNMT3A amino acid equivalent to the glutamic acid that is mutated in Dnmt3c rahu mutants (DNMT3A E861) is shown in red.