Marked Differences in Human Melanoma Antigen-Specific T Cell Responsiveness after Vaccination Using a Functional Microarray (original) (raw)
Figure 7
Antigen-Specific Profiles within Individual Patients
CD8+ lymphocytes were isolated from PBMCs from patients 10794, 10713, 10770, 10757, and 10742, and incubated on a pMHC functional cellular microarray containing anti-IFNγ co-spotted with several different peptide-MHC (HLA-A2) complexes. These included melanoma-associated antigens gp100, MART-1, and tyrosinase, and viral antigens from cytomegalovirus, influenza virus, and Epstein-Barr virus (gp100 209–2M, MART1 M26, tyrosinase 370D, CMV pp65, influenza MP, and EBV BLF, respectively). The resulting IFNγ responses varied by antigen-specific T cell population.