Dysfunctional KEAP1–NRF2 Interaction in Non-Small-Cell Lung Cancer (original) (raw)
Figure 6
Mutant KEAP1 Protein Is Unable to Suppress NRF2 Activity
(A) Repression activity of the KEAP1 mutants was monitored by a luciferase reporter assay. Wild-type and mutant KEAP1 cDNA constructs were transfected onto H838 cells stably expressing ARE luciferase reporter. Data represent mean ± SD (n = 3).
(B) Silencing of NRF2 by siRNA in A549 cells downregulated the expression of NRF2-dependent genes. A nonspecific siRNA (NS siRNA) was used as control.
(C) Inhibition of KEAP1 expression by siRNA in BEAS2B cells upregulated the expression of NRF2-dependent genes.