Analysis of Germline GLI1 Variation Implicates Hedgehog Signalling in the Regulation of Intestinal Inflammatory Pathways (original) (raw)
Figure 7
Cytokine Analysis of Gli1+/lacZ and WT Mice after DSS Treatments Demonstrates Robust Pro-inflammatory Cytokine Activation
(A, B) Cytokine and chemokine expression normalized to GAPDH is plotted on the _y_-axis for Gli1+/lacZ mice, and on the _x_-axis for WT animals. Gene expression levels that are statistically different are shown with stars. The dotted diagonal trend line indicates identical expression levels between WT and Gli1+/lacZ mice. (A) Cytokine and chemokine expression in WT and Gli1+/lacZ animals (n = 3) without DSS treatment. Cytokine and chemokine expression is low and very similar between WT and Gli1+/lacZ animals.
(B) Cytokine expression in WT and Gli1+/lacZ animals (n = 4) after 6 d of 3% DSS treatment. Many pro-inflammatory cytokines and chemokines are significantly more highly expressed in Gli1+/lacZ animals compared to WT. Note the difference in axes in panels (A) and (B).
(C) Fold change of cytokine expression from baseline to inflamed for WT and Gli1+/lacZ animals. We detect dramatic up-regulation of Th17 and Th1 pathway cytokines and chemokines in Gli1+/lacZ animals. *, p < 0.05; **, p < 0.01 by the Student's _t_-test.