Quantitative High-Throughput Screen Identifies Inhibitors of the Schistosoma mansoni Redox Cascade (original) (raw)
Figure 5
Example time-course plots derived from the primary screen, followed to the right by the corresponding concentration-response curves.
(A, B) Non-fluorescent inhibitors, compounds 3 and 14; the decrease in fluorescence is inhibited at increasing compound concentrations while the starting fluorescence remains constant. (C) An example of an apparent activator (Ofloxacin): a highly fluorescent compound which appears to be reacting with one or more components of the assay. Four concentrations out of seven or fifteen are plotted in each of the time course plots for clarity; ▪, 3.2 nM; ▴, 16 nM; □, 10 µM; ▵, 50 µM.