Schistosoma mansoni Tegument Protein Sm29 Is Able to Induce a Th1-Type of Immune Response and Protection against Parasite Infection (original) (raw)
Figure 2
Identification of Sm29 on skin-stage schistosomula tegument by Western blot.
Two micrograms of purified skin-stage schistosomula tegument was applied onto 12% SDS-PAGE and transferred to a nitrocellulose membrane by western blot. After that, the membrane was probed with serum from rSm29 vaccinated mice or naïve animals diluted 1∶500 in TBST. Arrow indicates the native Sm29. The molecular mass markers, from top to bottom, are: 97, 66, 45, 30, 20.1, and 14.4 kDa.