Phenotypic Screen of Early-Developing Larvae of the Blood Fluke, Schistosoma mansoni, using RNA Interference (original) (raw)
Figure 3
Additional S. mansoni sporocyst length measurements 7 days post-dsRNA treatments.
Graphic representation of sporocyst lengths (µm) after 7 days post-dsRNA treatments, generated from 3 independent experiments covering an additional group of sporocyst-expressed genes. Larva lengths are represented by a dot scatter plots with the median length for each sporocyst treatment group shown as a short horizontal bar. Calculated medians for each target dsRNA group were compared to both GFP-dsRNA (green dots) and no-dsRNA (blank, blue dots) control median values. For each experiment, controls are the first 2 scatter plots shown between the solid red and black vertical lines, followed by the 2 dsRNA treatments that showed significant phenotypic differences in all 3 experiments (marked with *; GST26 and SOD) when compared to both controls. Black scatter plots represent dsRNA-treated sporocysts whose median length measurements exhibited inconsistent differences when compared to both GFP and blank controls. Of this latter group (TPx1/2, fibrillarin, GST28, GPx, TPx2, TPx1, and SPO1), asterisks denote those individual replicates that were significantly different from controls. Each experiment was analyzed using Mann-Whitney _U_-test, *_P_≤0.05.