Phenotypic Screen of Early-Developing Larvae of the Blood Fluke, Schistosoma mansoni, using RNA Interference (original) (raw)
Figure 4
Localization of rhodamine-labeled-dsRNA in S. mansoni larvae 7 days post-exposure.
Brightfield (A) and fluorescent (B) photomicrographs showing S. mansoni sporocysts and localization of rhodamine-dsRNA taken up after 7 days of labeled dsRNA exposure, respectively (100×). Arrowheads indicate rounded epidermal plates that were shed from miracidia during transformation to sporocysts. (B) Fluorescent images show the different levels of dsRNA penetrance within the same treatment and the same population. (C) The higher magnification (400×) illustrating the heterogeneity of dsRNA uptake within individual sporocysts in a given population including excretory ducts/flame cells (FC), cells within the parenchyma (PC), and tegument (T).