Transplacental Transmission of Leishmania infantum as a Means for Continued Disease Incidence in North America (original) (raw)
Figure 1
Disseminated L. infantum infection in pups born to an infected female.
Tissue samples from target organs, as indicated, were collected at necropsy. DNA was isolated from 1 g of tissue using the QIAGEN DNA isolation kit. DNA was then analyzed via quantitative RT-PCR for the presence of L. infantum kinetoplast DNA. Data indicates detection of parasites in each organ and the number of L. infantum kinetoplast genome copies for (A) tissues from neonatal pups, 1–8, euthanized within 24 hours of birth, and (B) pups A–D euthanized at 3 months of age and the dam.