Transplacental Transmission of Leishmania infantum as a Means for Continued Disease Incidence in North America (original) (raw)
Figure 2
_L. infantum_-specific CD4+ T cell proliferative responses in vertically-infected pups.
Peripheral blood mononuclear cells (PBMC) were isolated from blood samples from neonatal pups (4–7), 3-month-old pups (A–D) and the dam. PBMC were stained with CFSE and cultured in the presence of L. infantum freeze-thawed antigen. PMBC were harvested at day 7 and the CD4+ T cell proliferation response was assessed via flow cytometry. Shown are the percentages of proliferating CD4+ T cells in response to L. infantum antigen.