Negative Regulation of Schistosoma japonicum Egg-Induced Liver Fibrosis by Natural Killer Cells (original) (raw)
Figure 3
Injection of poly I∶C further activates NK cells in S. japonicum infected mice.
_S. japonicum_-infected mice were injected intraperitoneally with poly I∶C (0.5 µg/g) since week 5 post-infection. (A–D) At various time points, hepatic MNCs were isolated and subjected for flow cytomery analysis. A representative FACS analysis of NK1.1 and CD3 was shown in (A). The percentage of NK cells among hepatic MNCs and the total number of NK cells are summarized in (B) and (C), respectively. The percentage of CD69+ NK cells among total NK cells was calculated and represented in (D). Data were presented as mean ± SEM (n = 5 for each group). *, P<0.05 versus corresponding saline-treated group. (E) Mice were sacrificed at week 6 post-infection, and IFN-γ expression by hepatic NK cells (CD3-NK1.1+) was examined by flow cytometry. (F) Hepatic MNCs from control IgG or anti-ASGM1 pretreated mice (denoted as “MNCs” and “MNCs without NK cells”, respectively) were incubated with poly I∶C (100 µg/ml) or saline for 48 hours. IFN-γ secretion in the supernatant was measured by ELISA. Data were presented as mean ± SEM; *, P<0.05.