Replacing a Native Wolbachia with a Novel Strain Results in an Increase in Endosymbiont Load and Resistance to Dengue Virus in a Mosquito Vector (original) (raw)
Figure 2
Inhibition of dengue infection in the midgut of MTB mosquitoes.
(A) At 4, 7 and 10 dpi with a blood meal containing DENV-2, mosquito midguts were collected, and the number of genome copies of the DENV-2 genome was determined by qRT-PCR using primers for the NS5 gene; the results were normalized to the Ae. polynesiensis RPS6. Lines indicate the median of the ten biological replicates. Significance was determined using a Mann-Whitney U test. (B) At 7 dpi through a blood meal, mosquito midguts were collected and fixed, and the viral antigen was detected using a mouse anti-Dengue complex monoclonal antibody by indirect fluorescence assay (IFA). One representative midgut from each category is shown. APM mosquitoes fed with dengue-infected or -uninfected blood meal was used as positive (POS.) and negative (NEG.) control, respectively. +, dengue-positive; −, dengue-negative.