Replacing a Native Wolbachia with a Novel Strain Results in an Increase in Endosymbiont Load and Resistance to Dengue Virus in a Mosquito Vector (original) (raw)
Figure 3
In vitro assay of DENV-2 transmission by MTB and APM mosquitoes.
At 14 days after a blood meal containing DENV-2 or infection through intrathoracic injection, the wings and legs were removed, and the proboscis of each mosquito was inserted into a feeding solution for 90 min. Solution from each mosquito was analyzed for infectious DENV-2 by plaque assays. Lines indicate the log of the median values. Twenty eight and eight biological replicates were used in oral feedings and intrathoracic injection assays, respectively.