Targeting Echinococcus multilocularis Stem Cells by Inhibition of the Polo-Like Kinase EmPlk1 (original) (raw)
Figure 5
Long-term BI 2536 effects on E. multilocularis metacestode vesicles.
Metacestode vesicles were treated for 21 days with different concentrations (10, 25, 50 and 100 nM) of BI 2536, followed by three days of recovery without inhibitor. On day three of recovery, EdU labeling (5 h, 50 µM EdU) was carried out and labeled cells were microscopically counted. (A) Microscopic images of the GL of BI 2536 treated (10, 25, 50, 100 nM) and non-treated E. multilocularis metacestode vesicles, stained with EdU. Bar represents 25 µm. Blue, nuclear staining, DAPI; red, EdU staining. (B) EdU-positive cell counts per mm2 of GL. ‘Control’ indicates vesicles cultivated in A4 medium alone. ‘Mock’ indicates the DMSO control. ***(p<0.001) (Student's t-test). Please note that the specific examples shown in (A) display slightly different total cell numbers but that the overall cell numbers in metacestode vesicles were not altered by drug treatment, as already outlined in [14] for HU treatment.