Identification of microRNA Genes in Three Opisthorchiids (original) (raw)
Fig 4
Upper: Scheme of primer target site positions in clusters: (A and B) miR-71a/2, (C) miR-71b/2.
Grey rectangles mean miRNA precursors, blue, purple and read rectangles—mature miRNAs; for->, for1->, for2-> and <-rev indicate primers and their directions. Lower: electrophoretogram of PCR products generated out of the cluster genomic regions. Designations: C-—negative control, M—lengths marker. DNA templates: Cs—C. sinensis, Of—O. felineus, Ov—O. viverrini. Primers used: (D) clust1-for1 and clust1-rev; (E) clust1-for2 and clust1-rev; (F) clust2-for and clust2-rev; G) Cl1-F and Cl2-r. The original electrophoretogram photographs are presented in S1 Fig.