Csseverin inhibits apoptosis through mitochondria-mediated pathways triggered by Ca2 + dyshomeostasis in hepatocarcinoma PLC cells (original) (raw)
Fig 2
Overexpression of _Cs_severin inhibits the apoptosis of PLC cells.
Apoptosis was analyzed by flow cytometry using an Annexin PE/7-AAD staining kit. After spontaneous apoptosis was induced by serum starvation for 48 h, pEZ-LV203-_Cs_severin PLC cells were stained with Annexin PE and 7-AAD and subsequently quantified by flow cytometry analysis. pEZ-LV203 PLC and PLC cells were used as controls.