Structural and Functional Analyses of Five Conserved Positively Charged Residues in the L1 and N-Terminal DNA Binding Motifs of Archaeal RadA Protein (original) (raw)
Figure 6
ssDNA binding.
The 5′-biotinylated (dT)50 oligonucleotide (10 µM in nucleotides) was first injected into BIAcore SA sensor chips. Wild-type or point mutant _Sso_RadA protein (1, 5, 10 µM) was passed over the chip at 25°C. Curves represent responses with the background subtracted. Binding signal was not detected when solutions that did not contain _Sso_RadA protein were injected.