A Multicassette Gateway Vector Set for High Throughput and Comparative Analyses in Ciona and Vertebrate Embryos (original) (raw)
Figure 3
Subcellular localisation of tagged proteins expressed from pSP72-pFOG::RfA vector series.
A, B, F, G epifluorescence pictures of embryos expressing GAP-43 venus (A), Histone2B-venus (B), venus-GATAa (F) and GATAa-HA (G). F and G show the same embryo co-electroporated with pSP72-pFOG::Venus-B1-GATAa-B2 and pSP72-pFOG::B1-GATAa-B2-HA. C, D, E projections of confocal stacks of images from embryos either electroporated (C, E) or microinjected with mRNA (D) and expressing ensconsin-3GFP (C, D), or ci-aurora kinase (E). Due to the mosaic expression of the transgene, some cells express the marker at a lower level (C, F, G).