A Multicassette Gateway Vector Set for High Throughput and Comparative Analyses in Ciona and Vertebrate Embryos (original) (raw)
Figure 9
Chordate expression constructs are suitable for mammalian cells and zebrafish embryos.
A) Transfection in mammalian cells of the indicated murine enhancers. The Y-axis shows the percentage of activity (% of LacZ positive cells) of tested Gateway-cloned enhancers relative to a control CMV-lacZ construct. The left most two columns were obtained in independent experiments from the other columns, restricted to Hela cells. B-D) Transient transgenesis by microinjection into zebrafish eggs of the indicated constructs. Left panel, lateral view, right panel, dorsal view, of 30 hour post-fertilization stage zebrafish embryos. No change in the intensity or localisation is observed when Gateway expression constructs are used (B, D) instead of pCS2+-based constructs (C). The numbers indicate the proportion of embryos with the shown lacZ or GFP staining.