Common Human Cancer Genes Discovered by Integrated Gene-Expression Analysis (original) (raw)
Figure 3
CLU and BID immunostaining in tumor and normal tissues.
Positive cytoplasmic staining of CLU presents on both tumor and normal cells in lung, breast, colon, and ovary tissue (A to D). CLU expression decreased in lung cancer (A, upper level) in comparison with normal lung (A, lower level). Both normal breast (B, lower level) and ovary (D, lower level) show middle to strong staining in cytoplasm, and middle to weak staining in breast (B, upper level) and ovarian cancer (D, upper level). Much less positive clusterin staining present in both colon tumor (C, upper level) and normal tissues (C, lower level). BID shows strong cytoplasmic and nuclear staining in lung cancer (E, upper level) and weak nuclear staining in normal lung epithelium (E, lower level). Increased strong nuclear and cytoplasmic staining is seen in breast tumor (F, upper level) when compared with normal breast tissue (F, lower level). In colon cancer, BID shows strong cytoplasmic and nuclear staining in tumor cells (G, upper level), while less positive BID staining was found in normal colon tissue (G, lower level). Semiquantitative analysis of CLU and BID immunoreaction in tumors and normal tissues (H). Most of samples from different types of cancer have strong, high percentage of BID immunoreaction, and normal tissues only show middle to low level staining; while CLU tends to down-regulated in tumors (H). High = score 10–12, middle = score 6–9, and low = score 1–5. Left column in each panel is at low power (100X) and right column in each panel is at high power (400X).